munc13-1 polyclonal  (Synaptic Systems)

Journal: bioRxiv

Article Title: Loss of postsynaptic NMDARs drives nanoscale reorganization of Munc13-1 and PSD-95

doi: 10.1101/2024.01.12.574705

Figure Lengend Snippet:

Article Snippet: Polyclonal rabbit anti-Munc13-1 , Synaptic Systems 126103 , AB_887733 , 0.5 mg/mL , 1:250.

Techniques: Concentration Assay

Journal: Neural Plasticity

Article Title: Diacylglycerol Signaling Underlies Astrocytic ATP Release

doi: 10.1155/2011/537659

Figure Lengend Snippet: The Munc13-1 protein is expressed in cultured astrocytes and reduction of Munc13-1 expression reduces astrocytic ATP release. (a) Immunocytochemistry for Munc13-1 in cultured astrocytes demonstrates expression of Munc13-1 in these cells: (green: Munc13-1, blue: DAPI). (b) Western blotting for Munc13-1 demonstrates a band at the expected size (~200 kD). Gel lanes were loaded with 60 μ g of cultured mouse astrocyte protein or 15 μ g of protein extracted from cultured mouse cortical neurons. (c) RT-PCR with primers designed against Munc13-1 of whole mouse brain mRNA and mRNA extracted from cultured astrocytes demonstrated a band for Munc13-1 at the expected size. Sequencing of these bands confirmed the presence of Munc13-1 in cultured astrocytes. (d) siRNAs designed against mouse Munc13-1 reduce the expression of Munc13-1 in transfected mouse astrocytes as measured by Western blot. Optical density of the Munc13-1 signal was standardized against the housekeeping protein GAPDH (scrambled siRNA n = 4, Munc13-1 siRNA = 4, P = 0.05). (e) The response of cultured astrocytes to OAG during one imaging session was measured for cultured astrocytes transfected with either scrambled control siRNAs or siRNAs directed against the Munc13-1 mRNA. The percentage of cells responding to OAG was significantly reduced after transfection with Munc13-1 siRNA (scrambled siRNA n = 6, Munc13-1 siRNA = 9; P = 0.017).

Article Snippet: To localize Munc13-1 protein, coverslips were incubated in affinity-purified rabbit polyclonal anti-Munc13-1 (1 : 100, Synaptic Systems) in blocking buffer for 48 hours at 4°C followed by the secondary antibody (goat antirabbit IgG-Alexa 588, 1 : 500, Invitrogen) for 1 hour at room temperature.

Techniques: Cell Culture, Expressing, Immunocytochemistry, Western Blot, Reverse Transcription Polymerase Chain Reaction, Sequencing, Transfection, Imaging

Journal: eLife

Article Title: Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones

doi: 10.7554/eLife.79077

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Munc13-1 (rabbit polyclonal) , SySy , Cat# 126103; RRID: AB_887733 ; LAC: A72 , IF (1:500); Western blot (WB) (1:1000).

Techniques: Recombinant, Plasmid Preparation, Immunofluorescence, Western Blot, Software, Generated